ILLUMINA SEQUENCING Whole-Genome Chromatin IP Sequencing (ChIP-Seq) Illumina ChIP-Seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify binding sites of DNA-associated proteins. Illumina ChIP-Seq technology precisely and cost-effectively maps global binding sites for a protein of interest

INTRODUCTION Transcription factors and other chromatin-associated proteins are essential phenotype-influencing mechanisms. Determining how proteins interact with DNA to regulate gene expression is essential for fully understanding many biological processes and disease states. This epigenetic information is complimentary to genotype and expression analysis. Traditional methods have successfully identified transcription factor binding sites and specific DNA-associated protein modifications and their roles in regulating specific genes, but these experiments are limited in scale and resolution. The powerful Illumina Whole-Genome Chromatin IP Sequencing (ChIP-Seq) application allows researchers to easily expand the scale of their studies to identify binding sites across the entire genome simultaneously with high resolution and without constraints. Specific DNA sites in direct physical interaction with transcription factors and other proteins can be isolated by chromatin immunoprecipitation (ChIP). ChIP produces a library of target DNA sites that a given factor was bound to in vivo. The revolutionary Solexa Sequencing technology is an ideal method to identify isolated DNA sites from ChIP. This massively parallel sequence analysis in the context of easy access to whole-genome sequence databases has made analyzing the interaction pattern of any protein with DNA, or the pattern of any epigenetic chromatin modifications, across the entire genome fast and cost-effective. The Illumina Genome Analyzer determines the sequences of ChIP-isolated DNA fragments to identify and quantify the sites bound by a protein of interest. ChIP-Seq technology supports virtually unconstrained selection of any ChIP-able protein and modifications HIGHLIGHTS OF ILLUMINA CHIP-SEQ